World Library  


Add to Book Shelf
Flag as Inappropriate
Email this Book

European Occupational Health Series : 1994-97; Prevention in Primary Care, Centre for Environmental Health, Volume 123: Environmental Health Services in Europe

By Andeas Kappos, Dr.

Click here to view

Book Id: WPLBN0000021265
Format Type: PDF eBook
File Size: 0.3 MB
Reproduction Date: 2005

Title: European Occupational Health Series : 1994-97; Prevention in Primary Care, Centre for Environmental Health, Volume 123: Environmental Health Services in Europe  
Author: Andeas Kappos, Dr.
Volume:
Language: English
Subject: Health., Public health, Wellness programs
Collections: Medical Library Collection, World Health Collection
Historic
Publication Date:
Publisher: World Health Organization

Citation

APA MLA Chicago

Dr, A. K. (n.d.). European Occupational Health Series : 1994-97; Prevention in Primary Care, Centre for Environmental Health, Volume 123. Retrieved from http://hawaiilibrary.net/


Description
Medical Reference Publication

Excerpt
Villus and crypt cells were removed from rabbit ileum by a modification of a method previously described. Villus cells were isolated by vibration of everted bowel segments in citrate buffer, and crypt cells were removed by the expansion of these segments with air and sin~ultaneous vibration in citrate buffer. Several culture media were tested for the maintenance and/or culture of these cells. Two defined media, Eagle's minimum and medium 199, were unsatisfactory and led to rapid cell death. The presence of whole serum was beneficial and both cell types could be maintained for several hours when homologous rabbit serum was employed. Initially 7030 g/, of villus and crypt cells were viable in homologous serum, and the effect of cholera toxin on cell viability was studied by incubating the cells with peptone dialysate supernatant (PSUP) toxin from Vibrio cholerae for 4 h. PSUP toxin reduced the viability of both villus and crypt cells compared with control preparations, as measured by the uptake of trypan blue ; cell death was accelerated with time. A purified, diarrhoea-inducing cholera toxin also reduced the viability of these cells and the results were comparable to those with PSUP toxin. The effect was usually immediate, and a significant reduction in viability occurred within 1 hour of the incubation of cells with toxin. The toxin was heat-labile (60°C/30 min) and nondialysable, and its cytotoxic activity could be completely nelitralized with cholera antitoxin. Dose-response studies indicated that as little as 0.0275 equivalent units of ligated ileal loop toxin were active in this system. In neither human nor experimental animal cholera is the integrity of the ileal epithelium affected by contact with the exotoxins formed by the cholera vibrio in the lumen of the bowel (16). Detailed studies of experimental canine infection during the first 20 h of the disease have also indicated that the epithelium remains intact. Other changes in the bowel tissue are consistent with the inference that hypersecretion, resulting from the enterotoxininduced movement of water and ions from the tissues into the lumen of the bowel, occurs mainly ' in the crypt cells, so that the cholera stool is produced primarily in the crypts (6).

Table of Contents
NA

 

Click To View

Additional Books


  • Bulletin of the World Health Organizatio... (by )
  • World Health Organization : (Emro) Regio... (by )
  • Health Organisation, Malaria Comission; ... (by )
  • World Health Organization : Year 1994, C... (by )
  • World Health Organization : Year 1995 ; ... (by )
  • World Health Organization : Year 1989 ; ... (by )
  • World Health Organization : Technical Re... (by )
  • World Health Organization : Year 1993 : ... (by )
  • World Health Organization Publication : ... (by )
  • World Health Organization : Year 1999 ; ... (by )
  • World Health Organization Drug Informati... (by )
  • European Occupational Health Series : Ye... (by )
Scroll Left
Scroll Right

 



Copyright © World Library Foundation. All rights reserved. eBooks from Hawaii eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.