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Production of Proinflammatory Mediators by Indoor Air Bacteria and Fungal Spores in Mouse and Human Cell Lines

By Huttunen, Kati

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Book Id: WPLBN0000086773
Format Type: PDF eBook
File Size: 0.5 MB
Reproduction Date: 2005
Full Text

Title: Production of Proinflammatory Mediators by Indoor Air Bacteria and Fungal Spores in Mouse and Human Cell Lines  
Author: Huttunen, Kati
Volume:
Language: English
Subject: Government publications, United Nations., United Nations. Office for Disarmament Affairs
Collections: Government Library Collection, Disarmament Documents
Historic
Publication Date:
Publisher: United Nations- Office for Disarmament Affairs (Unoda)

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Huttunen, K. (n.d.). Production of Proinflammatory Mediators by Indoor Air Bacteria and Fungal Spores in Mouse and Human Cell Lines. Retrieved from http://hawaiilibrary.net/


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Government Reference Publication

Excerpt
Excerpt: We compared the inflammatory and cytotoxic responses caused by household mold and bacteria in human and mouse cell lines. We studied the fungi Aspergillus versicolor, Penicillium spinulosum, and Stachybotrys chartarum and the bacteria Bacillus cereus, Pseudomonas fluorescens, and Streptomyces californicus for their cytotoxicity and ability to stimulate the production of inflammatory mediators in mouse RAW264.7 and human 28SC macrophage cell lines and in the human A549 lung epithelial cell line in 24-hr exposure to 105, 106, and 107 microbes/mL. We studied time dependency by terminating the exposure to 106 microbes/mL after 3, 6, 12, 24, and 48 hr. We analyzed production of the cytokines tumor necrosis factor-(Alpha) and interleukins 6 and 1(Beta) (TNF-(Alpha), IL-6, IL-1(Beta), respectively) and measured nitric oxide production using the Griess method, expression of inducible NO-synthase with Western Blot analysis, and cytotoxicity with the MTTtest. All bacteria strongly induced the production of TNF-(Alpha), IL-6 and, to a lesser extent, the formation of IL-1(Beta) in mouse macrophages. Only the spores of Str. californicus induced the production of NO and IL-6 in both human and mouse cells. In contrast, exposure to fungal strains did not markedly increase the production of NO or any cytokine in the studied cell lines except for Sta. chartarum, which increased IL-6 production somewhat in human lung epithelial cells. These microbes were less cytotoxic to human cells than to mouse cells. On the basis of equivalent numbers of bacteria and spores of fungi added to cell cultures, the overall potency to stimulate the production of proinflammatory mediators decreased in the order Ps. fluorescens > Str. californicus > B. cereus > Sta. chartarum > A. versicolor > P. spinulosum. These data suggest that bacteria in water-damaged buildings should also be considered as causative agents of adverse inflammatory effects. Key words: bacteria, cytokine production, fungi, inflammation, mold. Environ Health Perspect 111:85?92 (2003). [4 December 2002].

 

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