World Library  

Add to Book Shelf
Flag as Inappropriate
Email this Book

Production of Proinflammatory Mediators by Indoor Air Bacteria and Fungal Spores in Mouse and Human Cell Lines

By Huttunen, Kati

Click here to view

Book Id: WPLBN0000086773
Format Type: PDF eBook
File Size: 0.5 MB
Reproduction Date: 2005
Full Text

Title: Production of Proinflammatory Mediators by Indoor Air Bacteria and Fungal Spores in Mouse and Human Cell Lines  
Author: Huttunen, Kati
Language: English
Subject: Government publications, United Nations., United Nations. Office for Disarmament Affairs
Collections: Government Library Collection, Disarmament Documents
Publication Date:
Publisher: United Nations- Office for Disarmament Affairs (Unoda)


APA MLA Chicago

Huttunen, K. (n.d.). Production of Proinflammatory Mediators by Indoor Air Bacteria and Fungal Spores in Mouse and Human Cell Lines. Retrieved from

Government Reference Publication

Excerpt: We compared the inflammatory and cytotoxic responses caused by household mold and bacteria in human and mouse cell lines. We studied the fungi Aspergillus versicolor, Penicillium spinulosum, and Stachybotrys chartarum and the bacteria Bacillus cereus, Pseudomonas fluorescens, and Streptomyces californicus for their cytotoxicity and ability to stimulate the production of inflammatory mediators in mouse RAW264.7 and human 28SC macrophage cell lines and in the human A549 lung epithelial cell line in 24-hr exposure to 105, 106, and 107 microbes/mL. We studied time dependency by terminating the exposure to 106 microbes/mL after 3, 6, 12, 24, and 48 hr. We analyzed production of the cytokines tumor necrosis factor-(Alpha) and interleukins 6 and 1(Beta) (TNF-(Alpha), IL-6, IL-1(Beta), respectively) and measured nitric oxide production using the Griess method, expression of inducible NO-synthase with Western Blot analysis, and cytotoxicity with the MTTtest. All bacteria strongly induced the production of TNF-(Alpha), IL-6 and, to a lesser extent, the formation of IL-1(Beta) in mouse macrophages. Only the spores of Str. californicus induced the production of NO and IL-6 in both human and mouse cells. In contrast, exposure to fungal strains did not markedly increase the production of NO or any cytokine in the studied cell lines except for Sta. chartarum, which increased IL-6 production somewhat in human lung epithelial cells. These microbes were less cytotoxic to human cells than to mouse cells. On the basis of equivalent numbers of bacteria and spores of fungi added to cell cultures, the overall potency to stimulate the production of proinflammatory mediators decreased in the order Ps. fluorescens > Str. californicus > B. cereus > Sta. chartarum > A. versicolor > P. spinulosum. These data suggest that bacteria in water-damaged buildings should also be considered as causative agents of adverse inflammatory effects. Key words: bacteria, cytokine production, fungi, inflammation, mold. Environ Health Perspect 111:85?92 (2003). [4 December 2002].


Click To View

Additional Books

  • Value of Manufacturers' Shipments for In... (by )
  • Danish to English Dictionary (by )
  • Milk Production in Canada, Crop Rotation... (by )
  • The Grammar-School Dictionary, A Diction... (by )
  • Ensuring Food Quality and Safety and Fao... (by )
  • Mycotoxins of Growing Interest Fumonisin... (by )
  • Microbiology, A Text-Book of Microorgani... (by )
  • Breeding and Selection of Commercial Pou... (by )
  • The Novels and Letters of Jane Austen : ... (by )
  • The Foolish Dictionary (by )
  • Abhandlungen : Zur Neueren Geschichte (by )
  • How You Touched Me, You Will Never Know (by )
Scroll Left
Scroll Right


Copyright © World Library Foundation. All rights reserved. eBooks from Hawaii eBook Library are sponsored by the World Library Foundation,
a 501c(4) Member's Support Non-Profit Organization, and is NOT affiliated with any governmental agency or department.