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Journal of Applied Physics : Photobleaching-free infrared near-field microscopy localizes molecules in neurons

By J. Generosi, G. Margaritondo, M. Kropf, H. Hirling, S. Catsicas et al

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Book Id: WPLBN0002169480
Format Type: PDF eBook :
File Size: Serial Publication
Reproduction Date: 21 November 2008

Title: Journal of Applied Physics : Photobleaching-free infrared near-field microscopy localizes molecules in neurons  
Author: J. Generosi, G. Margaritondo, M. Kropf, H. Hirling, S. Catsicas et al
Volume: Issue : November 2008
Language: English
Subject: Science, Physics, Natural Science
Collections: Periodicals: Journal and Magazine Collection, Journal of Applied Physics Collection
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Publication Date:
Publisher: American Institute of Physics

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G. Margaritondo, M. Kropf, H. Hirling, S. Catsicas Et A, J. G. (n.d.). Journal of Applied Physics : Photobleaching-free infrared near-field microscopy localizes molecules in neurons. Retrieved from http://hawaiilibrary.net/


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Description: High-resolution detection of specific molecules in cells is a major challenge in biology. We show that infrared scanning near-field microscopy can detect the spatial distribution of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid–type glutamate receptor clusters on hippocampal neurons. The GluR2 subunits were labeled with the die Alexa 488 and high-resolution infrared micrographs were taken at 6.25 μm. The absence of photobleaching makes this approach suitable for a long-term observation and allows to localize different infrared-absorbing molecules over the complex background of other cell components.

 

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