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Physics of Fluids : Taylor dispersion in polymerase chain reaction in a microchannel

By Jinkee Lee, Elejdis Kulla, Anuj Chauhan, and Anubhav Tripathi

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Book Id: WPLBN0002169595
Format Type: PDF eBook :
File Size: Serial Publication
Reproduction Date: 2 September 2008

Title: Physics of Fluids : Taylor dispersion in polymerase chain reaction in a microchannel  
Author: Jinkee Lee, Elejdis Kulla, Anuj Chauhan, and Anubhav Tripathi
Volume: Issue : September 2008
Language: English
Subject: Science, Physics, Natural Science
Collections: Periodicals: Journal and Magazine Collection (Contemporary), Physics of Fluids Collection
Historic
Publication Date:
Publisher: American Institute of Physics

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Elejdis Kulla, Anuj Chauhan, And Anubhav Tripath, J. L. (n.d.). Physics of Fluids : Taylor dispersion in polymerase chain reaction in a microchannel. Retrieved from http://hawaiilibrary.net/


Description
Description: Polymerase chain reaction (PCR) is commonly used for a wide range of DNA applications such as disease detection, genetic fingerprinting, and paternity testing. The importance of PCR has led to an increased interest in performing PCR in a microfluidic platform with a high throughput while using very small DNA quantities. In this paper we solve convection-diffusion equations for the DNA and deoxynucleoside triphosphate (dNTP) under conditions suitable for PCR operation in a microchip. These include pressure driven flow accompanied by temporal temperature changes that lead to an amplification reaction, which is modeled as a first order reaction. The convection-diffusion-reaction equations are solved by using the method of multiple time scales to yield average equations that can be solved to obtain the long time evolution of the concentration profiles. The results obtained by solving the averaged equations agree well with full numerical solutions. The averaged equations are also solved to simulate the PCR to illustrate some interesting aspects of this operation in a microfluidic device. It is shown that insufficient nucleotide concentrations can lead to complete depletion of NTP at certain axial locations, which leads to termination of DNA amplification at these locations, resulting in formation of a plateau in DNA concentration.

 

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